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KMID : 0364819910290020075
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Korean Journal of Microbiology
1991 Volume.29 No. 2 p.75 ~ p.79
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A Yeast Chromosomal Gene that Induces Defective Interfering Particles of L-A dsRNA Virus in ski Host Cells
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Lee Hyun-Sook
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Abstract
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1
The yeast L-A virus (4.6 kb dsRNA genome) encodes the major coat protein and a "gag-pol" fusion minor coat protein that separately encapsidate itself and M,, a 1.8 kb dsRNA satellite virus encoding a secreted protein toxin (the killer toxin). The yeast chromosomal SKI genes prevent viral cytopathology by lowering the virus copy number. Thus, ski- mutants are is and cs for growth. We transformed a skit-2 virus-infested mutant with a yeast bank in a high copy cloning vector and selected the rare healthy transformants for analysis. One type of transformant segregated M-0 L-A-0 cells with high frequency. Elimination of the DNA clone from the ski2-2 strain eliminated this phenotype and introduction of the DNA clone recovered from such transformants into the parent skit-2 strain, or into ski3 or ski6 mutants gave the same phenotype. This killer-curing phenotype was due to the curing of the helper L-A dsRNA virus. The 6.5 kb insert only had this activity when carried on a high copy vector and in ski- cells (not in SKI* cells). This 6.5 kb insert acts as a mutagen on L-A dsRNA producing a high rate of deletion mutations.
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